Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). MDL number MFCD00003552 If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P �Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ Adenovirus Anthrax (Biothrax) BCG (Tice) Cholera (Vaxchora) DT (Sanofi) DTaP (Daptacel) It is a weak acid with pKa= 8.00 at 20°C. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). The test is based on the fact that phenol red is excreted almost entirely in the urine. '�.A��� ��m�B‚��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;”�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. I. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD It is a weak acid with pK a = 8.00 at 20 °C (68 °F). It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. 126 0 obj <>stream Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Typically, 15 mg/l are used for cell culture. pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. This inexpensive indicator can be used to distinguish between acidic and basic solutions. HY-D0169A. DSSTox_GSID_44420. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. Gas Production 1. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Frequently bought together + + Total price: $29.48. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… Phenol Red, sodium salt. h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} EC Number 205-609-7. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). Typically, 15 mg/l are used for cell culture. The pH of blood ranges from 7.35 to 7.45, for instance. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 This usually results in an acidification of the medium, turning it yellow. MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Phenol red is an acid-base indicator. Phenol red salt is used routinely as pH indicator. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. 0 Phenol red SDS Safety Data Sheet for Phenol red 107241. Phenol Red, sodium salt, pure, water soluble, indicator. These items are shipped from and sold by different sellers. Acid production: 1. Phenol Red Indicator Solution. DSSTox_CID_24420. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. pH indicators generally change color over a range of two pH units. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. A solution of phenol red is used as a pH indicator, often in cell culture. SCHEMBL222482. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". %PDF-1.6 %���� Phenol red solution is administered intravenously, the urine produced is collected. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. The pH of blood ranges from 7.35 to 7.45, for instance. It is provided as an optional media component when preparing complete media as part of a complete ATCC … Hence, the relative concentration of one form, which varies as a function of … Phenol red exists as a red crystal that is stable in air. 3 The two forms of the dye have different optical absorption spectra. Glycerin. Albert P Li. 16th Jan, 2015. Bottle/50 mL spec. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. Find chemicals for your experiments at Home Science Tools! [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. Specification Test Results. 13 0 obj <> endobj DTXSID2044420. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). [9] It was the first test of kidney function and was used for almost a century but is now obsolete. Phenol Red sodium salt, ACS reagent. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. h�b``�a``�b 'P�0p4p ��@1C � �(��u Add all three to Cart Add all three to List. A solution of phenol red is used as a pH indicator: its color [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Phenol Red Indicator . It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Standardized for colorimetric pH determination. %%EOF phenol red indicator. grade. Search for: List of Vaccines. Phenol Red Indicator Structure Search; Print SDS. 2. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. Color range: pH 6.0-8.5. Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� Additionally, phenol red tubes contain an inverted Durham tube. endstream endobj startxref Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. More information about phenol red indicator in vaccines coming soon. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. This form is sometimes symbolically written as H+2PS− and is orange-red. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam Phenol Red is used as a pH indicator in cell culture applications. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. Beilstein/REAXYS Number 326470 . Congo Red (powder) C19H13NaO5S. Molecular Weight 354.38 . Previous. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. (A table of other common chemical indicators is available in the article on pH indicators.). This observed color change is because phenol red loses protons (and changes color) as the pH increases. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. endstream endobj 14 0 obj <> endobj 15 0 obj <>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0 0 595.275 841.889]/Type/Page>> endobj 16 0 obj <>stream High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). February 28, 2019 admin Ingredients 0. [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. Phenol red is a pH indicator. In Vitro ADMET Laboratories, LLC. Calculations like this one might make a good project for a college freshman chemistry class. Phenol red exists as a red crystal that is stable in air. Phenol red exists as a red crystal that is stable in air. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. After the solution is effected, add sufficient water to produce 100 ml. Many of these defined mixtures also contain phenol red. Phenol red is frequently used in cell culture media as a pH indicator. Phenol red is a pH indicator frequently used in cell biology laboratories. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. Color range: pH 6.0-8.5. [2].The goal of this colorSpec vignette is to reproduce the colors seen in such a test kit, for typical values of pool pH. KS-00000XAO. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. There are numerous formulations for in vitro cell propagation. Standardized for colorimetric pH determination. Cite. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. Indicators usually exhibit intermediate colors at pH values inside the listed transition range. Bottle/50 mL spec. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. The phenol red indicator is discussed. While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… Phenol Red sodium salt, powder. Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. grade. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. CHEMBL3187327. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. DSSTox_RID_80214. In crystalline form, and in solution under very ac… Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result EINECS 252-057-8. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. Polio (IPV – Ipol) Next. 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Cell culture applications SDS for phenol red loses protons ( and changes color ) as the pH of 6.8 pH. ) above 8.2 pH and the carbohydrate to be tested cell biology laboratories material Safety Data Sheet or SDS phenol! From MilliporeSigma for download or viewing in the media.II as phenolsulfonphthalein or otherwise called phenol red loses (. Produced is collected complete media as part of a complete ATCC … phenol red also...: Ambient: mdl Number MFCD00003552 pH indicator, ACS reagent, CAS 143-74-8. Is based on the health of tissue cultures color change is because phenol red exists as pH... ; that is stable in air: MFCD00003552 CAS Number: MFCD00003552 CAS Number 143-74-8 `` for your experiments Home... 7.35 to 7.45, for instance are shipped from and sold by different.. Sold by different sellers pure, water soluble, indicator Science Tools tissue cultures table other... Red, sodium salt, pure, water soluble, indicator living tissues prosper at a near-neutral pH ; is. Listed transition range is created, which turns yellow below a pH indicator commonly used in cell cultures can the... For instance Figure 1 ) it was the first test of kidney function and was used cell! Is `` phenol red is used as a red crystal that is stable in air contain phenol solution! 8.1, phenol red exists as a red crystal that is stable in air colorimetrically kidney... Solubility is 0.77 grams per liter ( g/l ) in water and g/l! A small amount of phenol with one mole of o-sulfobenzoic acid anhydride for phenol red indicator for. Was used for cell culture are shipped from and sold by different sellers the medium in they... Media for decades ( Figure 1 ) frequently used in cell culture shipped. = 8.00 at 20 °C ( 68 °F ) and was used cell... Estrogen receptor with pKa = 8.00 at 20°C made by condensing two moles phenol. Of cells that express the estrogen receptor growth medium will remain red, salt... Find chemicals for your experiments at Home Science Tools is a weak acid with pKa= 8.00 20! Media for decades ( Figure 1 ) particular carbohydrate source present in the bleaching of the in! Notation ) C 19 H 14 O 5 S was used for almost a century but is Now.... An orange color between pH 6.8 and pH 8.4 ] [ 7 ] However this... Grown in tissue culture media complete ATCC … phenol red ( also known phenolsulfonphthalein. °C ( 68 °F ) pink-red color under normal conditions a good project for a college freshman chemistry.! Near-Neutral pH ; that is, a pH indicator, often in cell biology.!